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NAD+ 500mg

Essential Coenzyme Standard / Cellular Bioenergetics Regulator

$60.00$50.00

Grade: Research Grade / Analytical Standard

Purity: ≥99% (HPLC Verified)

Format: Lyophilized (Freeze-Dried) Powder

Unit Size: 500 mg

Product Overview

NAD+ 500mg (Nicotinamide Adenine Dinucleotide) is an essential, high-purity metabolic coenzyme found in all living cells, serving as a critical electron acceptor and cofactor in cellular oxidation-reduction reactions. NAD+ plays a central role in mitochondrial ATP generation via glycolysis, the Krebs cycle, and oxidative phosphorylation. Beyond energy metabolism, NAD+ functions as a obligate substrate for major enzymatic regulators, including Sirtuins (SIRT1–SIRT7), Poly(ADP-ribose) Polymerases (PARPs), and CD38/CD157 ectoenzymes.

This high-yield 500mg format provides research laboratories and multi-protocol facilities with an optimal, high-capacity standard for investigating cellular energy flux, age-related NAD+ depletion, mitochondrial quality control, and DNA damage repair pathways.

Key Research Features & Highlights

  • High-Capacity 500mg Mass: Designed for high-throughput screening, multi-target cellular assays, and extended experimental protocols without batch variance.

  • Sirtuin (SIRT) Pathway Activation: Serves as a required cofactor for deacetylase enzymes (SIRT1, SIRT3, SIRT6) governing histone modification, mitochondrial biogenesis, and metabolic gene expression.

  • PARP-Mediated DNA Repair: Acts as an essential substrate for PARP activation in response to oxidative stress, genomic instability, and strand breakage repair assays.

  • Mitochondrial Respiration & ATP Flux: Essential for evaluating electron transport chain efficiency, cellular redox ratios (NAD+/NADH), and mitochondrial bioenergetics in cultured cell lines.

  • Analytical Grade Purity (≥99%): Independently batch-tested via High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) to guarantee baseline consistency and reproducible research outcomes.

Primary Applications in Laboratory Research

  • Mitochondrial & Cellular Energy Kinetics: Investigating oxidative phosphorylation efficiency, ATP production dynamics, and mitochondrial dynamics (fission/fusion).

  • Longevity & Sirtuin Signaling: Studying epigenetic regulation, chromatin remodeling, and metabolic stress resistance via Sirtuin pathway activation.

  • Genomic Integrity & DNA Repair Mechanisms: Analyzing PARP-dependent DNA damage repair cascades under oxidative or radiation-induced cellular stress.

  • Age-Related Metabolic Decline Models: Evaluating NAD+ homeostasis, CD38-mediated NAD+ degradation, and cellular senescence markers in vitro.

Product Specifications

Attribute

Specification

Active Compound

Nicotinamide Adenine Dinucleotide (NAD+ / β-NAD)

Net Quantity

500 mg

Form

Lyophilized White to Off-White Powder

CAS Number

53-84-9

Molecular Mass

~663.43 g/mol

Molecular Formula

C21H27N7O14P2

Purity Standard

≥99% (HPLC verified)

Solubility

Highly soluble in Water, PBS, or Sterile Deionized Water

Storage & Handling Protocols

  • Unreconstituted (Lyophilized): Store frozen at or below -20°C in a dry, dark environment protected from thermal fluctuations, light, and humidity.

  • Reconstituted Solution: Maintain at 2°C – 8°C following dilution with sterile solvent (e.g., Sterile Water for Injection or PBS) and utilize rapidly within protocol parameters, as dissolved NAD+ is sensitive to aqueous degradation. Avoid repeated freeze-thaw cycles.

Mandatory Research & Legal Disclaimer

FOR LABORATORY RESEARCH USE ONLY. This compound is distributed exclusively for in vitro scientific research, educational, and analytical laboratory applications. It is not approved for human or animal consumption, medical therapy, diagnostic procedures, cosmetic application, or commercial drug formulation.